Objective: To analyze the financial input of disease prevention and control institutions in Wuhan from 2015 to 2021，so as to provide references for the reform，construction and development of the disease control system. Methods: Based on Wuhan health annual report and statistical yearbook data indicators from 2015 to 2021，comprehensive statistical analysis was made to analyze the problems of disease prevention and control institutions in Wuhan according to the field survey data and literature. Results: From 2015 to 2021，the establishment staff of disease prevention and control institutions was generally low; while the allocation of disease control institutions resources in the central-urban area was obviously lower than that in the remote urban area; the total financial input of disease control institutions has generally increased，but the growth rate was lower than that of the total input in health; the financial input of disease control institutions was volatile，and the growth rate was negatively correlated with the growth rate of GDP; the proportion of financial input in different types of disease control institutions was quite different. Conclusion: It is necessary to increase the number of establishment staff of disease control institutions，reasonably guide the allocation of disease control institutions resources in each district，sort out the responsibility orientation of disease control system at both urban and district levels，establish and perfect the financial investment guarantee mechanism，and explore the establishment of multi-channel sustainable financing mechanism.