Objective: To analyze the current status and accessibility of medical services in Lhasa and provide references on the allocation of medical resources in Lhasa. Methods: The allocation of medical resources in various districts and counties in Lhasa in 2020 was descriptively analyzed，and the accessibility of medical resources in Lhasa was evaluated by Gaussian two-step mobile search method and Getis-Ord Gi index. Results: Except Chengguan District，the number of beds and various health technicians in other districts and counties of Lhasa was generally lower than the national average. The utilization rate of sickbeds and the working efficiency of doctors in all districts and counties of Lhasa were relatively low，and there was still a large gap compared with the national average level. The overall accessibility of medical and health services in Lhasa was low，and the spatial differences among regions were large，showing a circular distribution structure (the central accessibility was high，and the accessibility gradually decreased to the surrounding areas，especially the northeast and northwest regions，which were the areas with the worst medical and health accessibility services in Lhasa. Conclusion: The medical resources in Lhasa were unevenly distributed and concentrated in the central region. Meanwhile，the medical services were of "low quality and low efficiency". It was suggested to improve the allocation standard of medical resources by relying on geographical classification; pay attention to regional characteristics and improve the accessibility of medical services; optimize the incentive mechanism and reform the human resource management system; innovate the Tibetan aid service mode，and explore the cross-regional medical service system.