Objective: It explores the differences of medical service pricing structure in the Yangtze River Delta region to provide a basis for promoting the macro adjustment of medical service prices. Methods: The catalogue of medical service items in the Yangtze River Delta in 2021 was collected. 300 representative medical service items were selected as the samples in this study. It vertically compared and analyzed the differences of the medical service price ratio in the Yangtze River Delta region based on the Kernel density estimation and the Meta-analysis. Results: In general，the pricing structure of various medical service items in each province or city shows a skew distribution，and the overall price level of Shanghai is higher than that of other provinces. Compared by different categories，the price ratio of comprehensive medical services projects in Zhejiang Province is comparatively large. The price ratio of medical technology and clinical diagnosis or treatment projects in Jiangsu Province and that of traditional Chinese medicine (TCM) diagnosis or treatment projects in Shanghai are comparatively small. Conclusion: The difference of overall price level among regions keeps pace with the economic development. Provinces should pay more attention to adjusting items with large differences in price ratio and maintain the consistency of price ratio. Shanghai should focus on adjusting the price ration of TCM diagnosis or treatment projects，to highlight important position and ensure the long-term and high-quality development of traditional Chinese hospitals.