The 41 cities in the Yangtze River Delta region were sampling and geographically divided by adopting cluster algorithm, and the geographic information system was used for visual description; the silhouette coefficient was used for fitting evaluation, and indicators such as medical expenses, health resources and service efficiency were selected to analyze the validity of the divisions. Taking Shanghai as the benchmark, the price ratio of each district was calculated. The geographical division of the Yangtze River Delta region is reasonable, and the geographical price ratio reflects the difference in economic costs, which is conducive to promoting the establishment of a reasonable price parity system for regional medical services in China.