Objective To establish a performance evaluation index system of maternal and child health care institutions to comprehensively evaluate the performance of the institutions in A Province. Methods Expert scoring method and factor analysis method was used to establish the index system. Linear efficacy coefficient method was used as subjective-objective combination method. Comprehensive scoring method was used in the performance evaluation of the institutions in A Province. Results A performance evaluation index system of maternal and child health care institutions was established. The evaluation results matched expert opinion and social evaluation. The sustainability of institutions in A Province should be focused on. Conclusion It is scientific to apply the subjective and objective combined weighting method to determine the weight of performance evaluation indicators in maternal and child health care institutions. Data quality, training, application of evaluation results, capacity for continuous development should be focused on.