Objective: From the investigation of rural grassroots medical staff recruitment, training, preparation and promotion of human resources integration, practice mentality and other aspects of the status quo, to explore the constraints of its talent team, and the corresponding strategies. Methods: Using multi-stage stratified random sampling to determine the research objects of the six cities in Shandong Province. And then we surveyed 1055 town doctors and 412 village doctors using questionnaire and interview，and analyzed the constraints of the construction of qualified personnel by SPSS statistical software and fish bone Chart. Results: The constraints include: the recruitment policy and the actual demand out of line, the lack of evaluation mechanism for training effectiveness, low income satisfaction, high working pressure and high medical liability risk, the establishment of management and curing, irrational title structure and separation, level medical institutions siphon grassroots medical institutions personnel, help is not targeted and so strong. Conclusion: we propose to increase the government’’s funding; actively implement the preparation of the record system, and strengthen the autonomy of personnel; strengthen personnel training and development mechanism; improve the composition of grass-roots titles; promote system and achieve synchronization evaluation; implement the high-level, transparent salary system and set up post subsidies; establish medical liability insurance system; implement the compact medical association, etc.